Advantages And Disadvantages Of The Serial Dilution Agar Plate Technique
Part C: UV Experiments Serial Dilutions and Viable Cell Counts The experiment Observing the Effects of Solar Ultraviolet Radiation on Cells shows that when cells are exposed to sunlight all, some, or none of them may be killed. Many experimental questions can be answered with qualitative answers like 'all, some, or none.' Other questions may require quantitative answers. For example, in the next experiment you will use the sensitive yeast strain to measure the intensity of solar UV radiation by measuring the fraction of cells exposed that survive. To get quantitative answers about yeast survival you must put a known numbers of viable (living) cells onto the agar plates and then count the number that remain after being exposed.
What Are Some Advantages And Disadvantages Of The Serial Dilution Agar Plate Technique
What are the advantages of the serial dilution agar plate procedure. Disadvantage: the method requires an incubation periods so it takes longer to get results. Serial dilution method for bacterial. Diluted bacteria samples then spread onto the agar plate by L-shaped. The advantages and disadvantages of serial.
You can determine the number of viable cells by counting the colonies that grow up on the agar growth medium in a Petri plate by assuming that each colony grows from a single viable cell. This is usually a reasonable assumption. Experiment: In the experiment that follows you will learn how to measure the number of viable cells on a Petri plate. You will be able to use this procedure whenever you need to measure the number of cells that survive an exposure to radiation or some other treatment. First you will estimate the number of cells in a liquid suspension in order to plate a reasonable number of cells. For this you will use one of the most sophisticated and sensitive optical instruments in existence, the human eye.
With surprisingly little practice you can learn to estimate the number of cells in a suspension by just looking at it. You can estimate cell density because of your eyes' fairly sharp threshold for observing turbidity (cloudiness). When viewed in a standard 13 100 mm glass tube, yeast suspensions of less than about 1 million cells per mL are not visibly turbid. Above this threshold density, the suspension is cloudy.
When you adjust the number of cells in a suspension until just barely visible, you obtain a suspension of known density (approximately 1 106 cells/ml). When you have a suspension that contains approximately 1 106 cells/ml, you will dilute it to get the right concentration for plating. You will make the dilutions in known steps so you can calculate the number of cells in each dilution tube. This procedure helps you plate a countable number of colonies.
The Quadrajet (Q-Jet) carburetor is better on a street/daily driven vehicle for a couple reasons. One is that the primary throttle bores are pretty small, so at part throttle, like most driving, you won't be using the entire carb, and hence you'll use less gas. Kekkaishi episode 1 sub indo mp4. Take for example a mechanical secondary Holley four barrel. At part throttle, all four barrels are open and spraying fuel. In a Q-Jet, only two barrels are open and spraying fuel.
One other reason is that it's a vacuum secondary carb. This means that the secondaries open a bit slower, so if you need to gun it, you'll have the power, but won't use all the fuel at once, saving you a bit. Again, take a Holley for example.
Go from 25% throttle to 100%. You go from four barrels spraying some fuel to all barrels spraying maximum capacity.
This could bog your engine down from too much fuel for too few RPMs if not tuned correctly, and will immediately use more fuel. On the other hand, the Q-Jet would open the secondaries slower, allowing the engine to come up into the higher RPMs it would need for the extra fuel. Say you go from 25% to 100% throttle.
You go from two barrels spraying some fuel to two barrels spraying maximum capacity, and then in addition, two bigger barrels spraying 25% capacity, 50% capacity, 75% capacity and if you stay in it long enough, 100% capacity. This would use progressively more and more fuel, so for a quick spurt, you would save some fuel you would have used with the Holley, say only going up to 50% capacity on the secondaries compared to the Holleys 100% capacity immediately. But, you do lose some responsiveness. This all still happens pretty quickly, as in within a split second for the Holley to go 100% compared to a Q-Jet taking 1 second or so to go fully open.
Other than that, the Q-Jet is basically on par with other carbs. They can be harder to tune, but if you know what you're doing, it will just take a bit more time and effort, not much. In a cruiser or daily driver, you'd be better off using a Q-Jet.
For a race car or weekend toy, just go for the Holley since you won't be driving it all that much, and they are easier to set up and tune for most people. Pacific, North American, South American, Eurasian, Persian subplate, African, Somalian Subplate, Austral-Indian, Antarctic Technically they are referred to as tectonic plates, between the 7 major tectonic plates they cover most of the earth's surface except for where a few smaller ones exist. Because they cover such a large area the borders of each plate won't correspond to any specific counrty or reference point that can easily be referred to as a particular plates location. Extremely generalised locations are stated below for the 7 largest techtonic plates. Pacific Plate - covers the majority of the Pacific Ocean 2. Antarctic Plate - covers Antarctica and much of the far Southern Ocean area 3. Indo-Australian Plate - stretches through Indonesia, Australia, New Zealand, Papua New Guinea and on to the Canary Islands 4.
Eurasian Plate - covers all of Europe and most of Asia except the Philippines 5. African Plate - covers Africa and much of the surrounding ocean 6. North America plate - Covers North America and Arctic regions 7. South America Plate - covers South America and a large portion of the southern Atlantic Ocean There are eight smaller plates that are still larger than most countries.